Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011;30(4):209-19.
doi: 10.3109/15513815.2011.572960. Epub 2011 Jun 20.

Comparative immunohistochemical study of multicystic dysplastic kidneys with and without obstruction

Claudia P Rojas  1 , Arnel K Urbiztondo, Jocelyn H Bruce, Maria M Rodriguez
Affiliations

Comparative immunohistochemical study of multicystic dysplastic kidneys with and without obstruction

Claudia P Rojas et al. Fetal Pediatr Pathol. 2011.

Abstract

Etiology of multicystic dysplastic kidney (MCDK) remains unknown. Not all cases are associated with obstruction. We compared by immunohistochemistry 17 cases of MCDK (10 cases with and seven without obstruction) to 17 controls and 20 fetal kidneys. TGF-β was negative in obstructive MCDKs and positive in nonobstructive MCDK. IGF2 was overexpressed in obstructive and underexpressed in nonobstructive MCDKs. PAX2, BCL-2, and β-catenin were expressed equally in obstructive and nonobstructive dysplasia. TGF-β and IGF2 work by different mechanisms in obstructive and nonobstructive MCDKs, but there are no differences among PAX 2, BCL-2, and β-catenin in obstructive versus nonobstructive dysplasia.

PubMed Disclaimer

Figures

FIGURE 1
FIGURE 1
(A) Obstructive MCDK. Notice the hypoplastic kidneys with the adrenal glands still at the upper poles (arrow). The aorta is centrally located and there are bilateral hydroureters. (B) Nonobstructive MCDK. The ureters are unremarkable and portions of the two umbilical arteries run parallel to the urinary bladder. The kidneys are bi-valved to demonstrate numerous small cysts scattered through the cortex and medulla. 101 × 67mm.
FIGURE 2
FIGURE 2
TGF-β staining. (A) Negative in obstructive MCDK. (B) Positive staining in interstitium and tubular epithelium of nonobstructive (syndromic) MCDK. (C) Negative staining in tubules including nephrogenic zone of fetus. (D) Focal staining in glomeruli of controls. 76 × 57 mm.
FIGURE 3
FIGURE 3
PAX2. (A) Positive staining in dysplastic tubular epithelium and proximal tubules in obstructive MCDK. (B) Nonobstructive MCDK, positive staining in dysplastic tubular epithelium and proximal tubules. (C) Strong staining in fetal kidney. (D) Over-expression of PAX2 in nephrogenic zone of immature kidney (less than 36 weeks). 76 × 57 mm.
FIGURE 4
FIGURE 4
BCL2 expression. (A) Positive cytoplasmic staining in dysplastic tubular epithelium of obstructive MCDK. (B) Similar positivity in non-obstructive MCDK. (C) Positive in nephro-genic zone of fetal kidney. (D) Minimal expression in the tubules of normal control (arrows). 76 × 57 mm.
FIGURE 5
FIGURE 5
Beta-catenin panel. (A) Membranous staining in dysplastic tubular epithelium of a case with obstructive MCDK. (B) Same pattern in a patient with nonobstructive MCDK. (C) Overexpres-sion in nephrogenic zone of fetal kidney. (D) Positive reaction in normal tubular epithelium of control. 76 × 57 mm.
FIGURE 6
FIGURE 6
IGF2 expression in (A) obstructive MCDK depicts strongly positive membranous and cytoplasmic staining. (B) Weaker membranous expression in non-obstructive MCDK. (C) Positive staining in nephrogenic zone of preterm kidney. (D) Positive normal control. 76 × 57 mm.
See this image and copyright information in PMC

References

    1. Gordon AC, Thomas DF, Arthur RJ, Irving HC. Multicystic dysplastic kidney: is nephrectomy still appropriate? J Urol. 1988;140:1231–1234. - PubMed
    1. Tian YC, Chen YC, Chang CT, Hung CC, Wu MS, Phillips A, Yang CW. Epidermal growth factor and transforming growth factor-beta 1 enhance HK-2 cell migration through synergistic increase of the matrix metalloproteinase and sustained activation of ERK signaling pathway. Exp Cell Res. 2007;313:2367–2377. - PubMed
    1. Winyard PJD, Risdon RA, Sams VR, Dressier GR, Woolf AS. The PAX2 transcription factor is expressed in cystic and hyperproliferative dysplastic epithelia in human kidney malformations. J Clin Invest. 1996;98:451–459. - PMC - PubMed
    1. Granata C, Wang Y, Puri P, Tanaka K, O'Briain DS. Decreased bcl-2 expression in segmental renal dysplasia suggests a role in its morphogenesis. BrJ Urol. 1997;80:140–144. - PubMed
    1. Matsell DG, Bennett T, Armstrong RA, Goodyer P, Goodyer C, Han VKM. Insulin-like growth factor (IGF) and IGF binding protein gene expression in multicystic renal dysplasia. J Am Soc Nephrol. 1997;8:85–94. - PubMed

MeSH terms

LinkOut - more resources