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. 2011 Aug 7;47(29):8277-9.
doi: 10.1039/c1cc12131f. Epub 2011 Jun 21.

Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine by a chemically designed peptide

Akiko Nomura  1 , Kaori Sugizaki, Hiroyuki Yanagisawa, Akimitsu Okamoto
Affiliations

Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine by a chemically designed peptide

Akiko Nomura et al. Chem Commun (Camb). .

Abstract

An artificial phosphopeptide recognized the difference between methylated and hydroxymethylated cytosines in DNA. The Sp1 zinc finger peptide substituted by phosphotyrosine effectively discriminated between 5-methylcytosine, 5-hydroxymethylcytosine ((hm)C) and unmethylated cytosine. The DNA recognition properties of the peptide differ from those of other chemicals that detect (hm)C.

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