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. 2011 Jul 18;7(14):2010-5.
doi: 10.1002/smll.201100511. Epub 2011 Jun 22.

Wet nanoscale imaging and testing of polymersomes

Giuseppe Battaglia  1 , Caterina LoPresti, Marzia Massignani, Nicholas J Warren, Jeppe Madsen, Simon Forster, Cvetilin Vasilev, Jamie K Hobbs, Steven P Armes, Somoyot Chirasatitsin, Adam J Engler
Affiliations

Wet nanoscale imaging and testing of polymersomes

Giuseppe Battaglia et al. Small. .
No abstract available

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Figures

Figure 1
Figure 1
A) Schematics of the biotinylated polymersome binding to a streptavidin surface. B) Confocal and STED imaging of 200 nm fluorescently (ATTO label)-labelled polymersomes immobilized by biotinylated PEG–phospholipid confirm the enhanced STED resolution by examining the intensity profile (scale bar = 500 nm).
Figure 2
Figure 2
A) Tapping-mode liquid AFM height image of biotinylated polymersomes immobilized by Biotinylated PEG-phospholipid onto Si substrate coated with streptavidin. B) The corresponding topographic 3D rendered image showing the distribution of polymersome heights. C) AFM-measured polymersome height versus diameter for Biotin–PMPC–PDPA and Biotin–PEG–DSPE immobilized PMPC–PDPA polymersomes. D) AFM- and DLS-measured particle size histograms.
Figure 3
Figure 3
A) Force spectroscopy mapping-mode schematic. B) FSM map overlaid onto topographic images to illustrate the spatial location of an adhesive event on the polymersome surface immobilized by biotin–PMPC–PDPA copolymers. Histograms of streptavidin surface alone (C) and polymersomes (D) illustrate the differences between nonspecific adhesion of the tip (single peak in C and first peak in D) and the specific adhesion event observed in D.
See this image and copyright information in PMC

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