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. 1990 Aug 27;1050(1-3):155-9.
doi: 10.1016/0167-4781(90)90158-x.

Translation initiation factor-dependent extracts from Saccharomyces cerevisiae

M Altmann  1 , S Blum, J Pelletier, N Sonenberg, T M Wilson, H Trachsel
Affiliations

Translation initiation factor-dependent extracts from Saccharomyces cerevisiae

M Altmann et al. Biochim Biophys Acta. .

Abstract

Translation initiation factor 4A- and 4E-dependent extracts were developed from Saccharomyces cerevisiae and used to study factor requirements for translation of individual mRNAs in vitro. Whereas all mRNAs tested required eIF-4A, mRNAs devoid of secondary structure in their 5' untranslated region did not require exogenous eIF-4E for translation. The latter included alfalfa mosaic virus RNA4, mRNA containing the untranslated region of tobacco mosaic virus RNA and mRNA containing part of the untranslated region of poliovirus RNA. Furthermore, initiation of translation on mRNAs containing part of the untranslated region of poliovirus RNA is most likely internal.

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