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. 1978 Oct;75(10):4873-6.
doi: 10.1073/pnas.75.10.4873.

DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor

M I Baldi, E Mattoccia, G P Tocchini-Valentini

DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor

M I Baldi et al. Proc Natl Acad Sci U S A. 1978 Oct.

Abstract

A purified system is described for the introduction of negative supercoils into simian virus 40 DNA. The system consists of histones H2A, H2B, H3, and H4, DNA-relaxing enzyme, and a purified factor from Xenopus laevis stage 6 oocyte nuclei. The nuclei are prepared en masse by the technique of F. Scalenghe, M. Buscaglia, C. Steinheil, and M. Crippa [(1978) Chromosoma 60, 299-308]. The supercoiled simian virus 40 DNA prepared by this method is indistinguishable from simian virus 40 supercoiled DNA prepared from infected monkey cells.

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References

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