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Comment
. 2011 Jul 5;108(27):10933-4.
doi: 10.1073/pnas.1107835108. Epub 2011 Jun 23.

RNA structure probing dash seq

Kevin M Weeks  1
Affiliations
Comment

RNA structure probing dash seq

Kevin M Weeks. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Overview of an RNA structure probing dash seq experiment. An RNA pool (Upper Left) is subjected to RNA structure probing to yield a chemical imprint of the initial structures (Left, colored structures). Red, yellow, and black spheres indicate nucleotides with high, medium, and low reactivities, respectively, toward structure probing. (Center) RNAs are then converted to double-stranded DNA molecules for NGS; not all processing steps shown are used in every NGS experiment. (Right) Sequencing yields a count of the original RNA modifications times the effects of all processing steps, which must be deconvoluted to recapitulate fully the results of the original probing step.
See this image and copyright information in PMC

Comment on

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