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. 1990 Oct;172(10):5686-9.
doi: 10.1128/jb.172.10.5686-5689.1990.

Pyrophosphatase is essential for growth of Escherichia coli

J Chen  1 , A Brevet, M Fromant, F Lévêque, J M Schmitter, S Blanquet, P Plateau
Affiliations

Pyrophosphatase is essential for growth of Escherichia coli

J Chen et al. J Bacteriol. 1990 Oct.

Abstract

The ppa gene for inorganic pyrophosphatase is essential for the growth of Escherichia coli. A recombinant with a chromosomal ppa::Kanr lesion and a temperature-sensitive replicon with a ppa+ gene showed a temperature-sensitive growth phenotype, and a mutant with the sole ppa+ gene under control of the lac promoter showed inducer-dependent growth. When the lacp-ppa mutant was subcultured without inducer, the pyrophosphatase level decreased, the PPi level increased, and growth stopped. Cellular PPi reached 16 mM about 6 h after growth arrest without loss of cell viability.

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