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. 2011 Sep;86(3):393-6.
doi: 10.1016/j.mimet.2011.06.008. Epub 2011 Jun 17.

Culture-independent multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae

Roger Dumke  1 , Enno Jacobs
Affiliations

Culture-independent multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae

Roger Dumke et al. J Microbiol Methods. 2011 Sep.

Abstract

A PCR approach for multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae directly from respiratory samples was developed and evaluated on 54 specimens from M. pneumoniae-positive pneumonia patients. The method resulted in a clearly identifiable MLVA type in all samples tested and can be used for MLVA without laborious isolation of the pathogen.

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