Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Oct;43(2):254-60.
doi: 10.1007/s11262-011-0635-3. Epub 2011 Jun 26.

Sequence analysis of S1 genes of infectious bronchitis virus isolated in Thailand during 2008-2009: identification of natural recombination in the field isolates

Tawatchai Pohuang  1 , Niwat Chansiripornchai, Achara Tawatsin, Jiroj Sasipreeyajan
Affiliations

Sequence analysis of S1 genes of infectious bronchitis virus isolated in Thailand during 2008-2009: identification of natural recombination in the field isolates

Tawatchai Pohuang et al. Virus Genes. 2011 Oct.

Abstract

During 2008-2009, fifteen field infectious bronchitis viruses (IBVs) were isolated from commercial chicken farms in Thailand. After sequencing of the complete S1 gene, phylogenetic analysis was performed and this found that the Thai IBV isolates were divided into three distinct groups, unique to Thailand (group I), QX-like IBV (group II), and Massachusetts type (group III). This finding indicated that the recent Thai IBVs evolved separately and that at least three groups of viruses are circulating in Thailand. The recombination analysis of the S1 gene demonstrated that the 5'-terminus of the group I was similar to isolate THA001 which was unique to Thailand, isolated in 1998 whereas the 3'-terminus was similar to the group II. Moreover, the analysis of the S1 gene of the group II showed that the 5'-terminus was similar to QXIBV, isolated in China whereas the remaining region at the 3'-terminus was similar to the Chinese strain JX/99/01. The results indicated that the recombination events occurred in the S1 gene between the field strains. Based on these facts, the field IBV in Thailand has undergone genetic recombination.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Phylogenetic tree based on the complete nucleotide sequences of the S1 gene between Thai IBV isolates, group I (filled diamond), group II (filled triangle), and group III (filled circle) and published sequences
Fig. 2
Fig. 2
Similarity plot of the S1 gene of the group I Thai IBV (represented by THA90151). Isolate THA80151 (filled triangle) and isolate THA001 (filled square) were used as putative parental strains when isolate THA90151 was queried. M41 (no fill) was used as an outlier sequence
Fig. 3
Fig. 3
Similarity plot of the S1 gene of the group II Thai IBV (represented by THA80151). QXIBV (filled triangle) and JX/99/01 (filled square) were used as putative parental strains when isolate THA80151 was queried. M41 (no fill) was used as an outlier sequence
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Parsons D, Ellis MM, Cavannagh D, Cook AKJ. Vet. Rec. 1992;131:408–411. doi: 10.1136/vr.131.18.408. - DOI - PubMed
    1. Animas BS, Otsuki K, Hanayama M, Sanekata T, Tsubokura M. J. Vet. Med. Sci. 1994;56:443–447. doi: 10.1292/jvms.56.443. - DOI - PubMed
    1. Gough RE, Randall CJ, Dagless M, Alexander DJ, Cox WJ, Pearson D. Vet. Rec. 1992;130:493–494. doi: 10.1136/vr.130.22.493. - DOI - PubMed
    1. Liu SW, Kong XG. Avian Path. 2004;33:321–327. doi: 10.1080/0307945042000220697. - DOI - PMC - PubMed
    1. Ziegler AF, Ladman BS, Dunn PA, Schneider A, Davison S, Miller PG, Lu H, Weinstock D, Slem M, Eckroade RJ, Gelb J., Jr Avian Dis. 2002;46:847–858. doi: 10.1637/0005-2086(2002)046[0847:NIBIPC]2.0.CO;2. - DOI - PubMed

Publication types