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. 2011 May;43(3):340-4.
doi: 10.4103/0253-7613.81502.

Studies on the activity of Cyperus rotundus Linn. tubers against infectious diarrhea

Poonam G Daswani  1 , S Brijesh, Pundarikakshudu Tetali, Tannaz J Birdi
Affiliations

Studies on the activity of Cyperus rotundus Linn. tubers against infectious diarrhea

Poonam G Daswani et al. Indian J Pharmacol. 2011 May.

Abstract

To study the antidiarrheal activity of the decoction of Cyperus rotundus Linn. tubers using representative assays of diarrheal pathogenesis and understand its mechanism of action.Antibacterial, antigiardial and antirotaviral activities were studied. Effect on adherence of enteropathogenic Escherichia coli (EPEC) and invasion of enteroinvasive E. coli (EIEC) and Shigella flexneri to HEp-2 cells was evaluated as a measure of effect on colonization. Effect on enterotoxins such as enterotoxigenic E. coli (ETEC) heat labile toxin (LT), heat stable toxin (ST) and cholera toxin (CT) was also assessed. The decoction showed antigiardial activity, reduced bacterial adherence to and invasion of HEp-2 cells and affected production of CT and action of LT. The decoction of C. rotundus does not have marked antimicrobial activity and exerts its antidiarrheal action by mechanisms other than direct killing of the pathogen.

Keywords: Bacterial adherence; Cyperus rotundus; HEp-2 cells; infectious diarrhea.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Antigiardial activity of the decoction of C. rotundus [C: Control, trophozoites in medium alone; M: trophozoites incubated in medium with metronidazole (10 μg/mL)]. Values represent mean ± standard error (n= 3) of percentage viable trophozoites relative to control (100%); ∗P < 0.05
Figure 2
Figure 2
Effect of the decoction of C. rotundus on bacterial colonization to HEp-2 cells: (a) Adherence of EPEC strain B170; (b) Invasion of EIEC strain E134; (c) Invasion of S. flexneri (C: Control, bacterial adherence/invasion to HEp-2 cells in medium alone; L1: adherence/invasion to HEp-2 cells in medium with 2.5 mg/mL lactulose; L2: adherence to HEp-2 cells in medium with 15 mg/mL lactulose in the competitive protocol). Values represent mean ± standard error (n = 3) of percentage adherence/invasion relative to respective control (100%); ∗P < 0.05
Figure 3
Figure 3
Effect of the decoction of C. rotundus on bacterial enterotoxins: (a) Production of heat labile toxin (LT) by E. coli B831-2 and its binding to GM1; (b) Production of cholera toxin (CT) by V. cholerae and its binding to GM1 (C: Control, toxin in medium alone; M1: LT in medium with 5 mM 2-mercaptoethanol; M2: CT in medium with 1 mM 2-mercaptoethanol; G: LT/CT in medium with 50 mM gallic acid). Values represent mean ? standard error (n = 3) of percentage production/binding relative to respective control (100%); ∗P < 0.05
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