[Serologic methods for the overall diagnosis of bovine herpes mamillitis]

Abstract

Developed were methods of seroneutralization, agar gel precipitation, and complement-fixation test (CFT) for the complex diagnosis of herpes mamillitis of herpes mamillitis in cattle. An agreement was found between the results obtained with the use of each method. Highest sensitivity and specificity possessed the seroneutralization reaction with which a highest percent of positively reacting sera was revealed (18.6). However, the CFT method could preferably be applied in mass investigations as it is more quickly and readily used as a screening test. By means of the agar gel precipitation and CFT methods the antigenic relatedness between Bovid herpesvirus 2 and the remaining representatives of the herpes virus group, and chiefly Human herpes virus 1, could be demonstrated.