Environmental factors associated with larval habitats of anopheline mosquitoes (Diptera: Culicidae) in irrigation and major drainage areas in the middle course of the Rift Valley, central Ethiopia

Abstract

Background & objectives: Larval control is an integral part of malaria vector management in Ethiopia and elsewhere. For effective larval control, a sound understanding of the factors responsible for spatio-temporal variation in larval production is essential. A study was thus conducted to characterize larval habitats of anopheline mosquitoes in irrigation and major drainage areas between Adami Tulu and Meki towns, in the middle course of the Ethiopian Rift Valley.

Methods: Aquatic habitats were sampled for anopheline larvae and the associated environmental variables (water temperature, turbidity, water current, water pH and other variables) were measured, characterized and analyzed.

Results: Microscopic identification of the late instars (III and IV) of anopheline larvae collected throughout the study period yielded nearly 47.6% Anopheles pharoensis, 32.1% An. arabiensis, 17.1% An. squamosus and only 3.2% of other species (An. coustani and An. cinereus). Larvae of the local malaria vectors, An. arabiensis and An. pharoensis were most abundantly sampled from sand pools and natural swamps, respectively. Logistic regression analysis detected four best predictor variables associated with larval abundance of malaria vector species. Thus, relative abundance of An. arabiensis larvae was significantly and inversely associated with aquatic vegetation and water current, whereas the relative abundance of An. pharoensis larvae was significantly and positively associated with water temperature and the presence of algae in the water bodies.

Conclusion: Dry season anopheline larval habitats such as riverine sand pools that are created and maintained by perennial water bodies and their associated water development projects need to be considered in vector control operations.