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. 2011 Apr;7(26):126-32.
doi: 10.4103/0973-1296.80670.

Sodium metabisulfite-induced polymerization of sickle cell hemoglobin incubated in the extracts of three medicinal plants (Anacardium occidentale, Psidium guajava, and Terminalia catappa)

Paul Chidoka Chikezie  1
Affiliations

Sodium metabisulfite-induced polymerization of sickle cell hemoglobin incubated in the extracts of three medicinal plants (Anacardium occidentale, Psidium guajava, and Terminalia catappa)

Paul Chidoka Chikezie. Pharmacogn Mag. 2011 Apr.

Abstract

Background: The exploitation and utilization of vast varieties of herbal extracts may serve as alternative measures to deter aggregation of deoxygenated sickle cell hemoglobin (deoxyHbS) molecules.

Objective: The present in vitro study ascertained the capacity of three medicinal plants, namely, Anacardium occidentale, Psidium guajava, and Terminalia catappa, to alter polymerization of HbS.

Materials and methods: Spectrophotometric method was used to monitor the level of polymerization of hemolysate HbS molecules treated with sodium metabisulfite (Na(2) S(2) O(5)) at a regular interval of 30 s for a period of 180 s in the presence of separate aqueous extracts of A. occidentale, P. guajava, and T. catappa. At time intervals of 30 s, the level of polymerization was expressed as percentage of absorbance relative to the control sample at the 180th s.

Results: Although extracts of the three medicinal plants caused significant (P < 0.05) reduction in polymerization of deoxyHbS molecules, the corresponding capacity in this regard diminished with increase in incubation time. Aqueous extract of P. guajava exhibited the highest capacity to reduced polymerization of deoxyHbS molecules. Whereas at t > 60 s, extract concentration of 400 mg% of A. occidentale activated polymerization of deoxyHbS molecules by 6.23±1.34, 14.53±1.67, 21.15±1.89, and 24.42±1.09%, 800 mg% of T. catappa at t > 30 s gave values of 2.50±1.93, 5.09±1.96, 10.00±0.99, 15.38±1.33, and 17.31±0.97%.

Conclusion: The capacity of the three medicinal plants to interfere with polymerization of deoxyHbS molecules depended on the duration of incubation and concentration of the extracts.

Keywords: A. occidentale; DeoxyHbS; P. guajava; Polymerization; T. catappa.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Change in absorbance of erythrocyte haemolysate of HbSS genotype in the presence of aqueous extract of A. occidentale
Figure 2
Figure 2
Change in absorbance of erythrocyte haemolysate of HbSS genotype in the presence of aqueous extract of P. guajava
Figure 3
Figure 3
Change in absorbance of erythrocyte haemolysate of HbSS genotype in the presence of aqueous extract of T. catappa
See this image and copyright information in PMC

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