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. 2011:2011:615032.
doi: 10.1155/2011/615032. Epub 2011 Jun 2.

Isolation and Characterization of a Phosphate-Solubilizing Halophilic Bacterium Kushneria sp. YCWA18 from Daqiao Saltern on the Coast of Yellow Sea of China

Fengling Zhu  1 , Lingyun Qu, Xuguang Hong, Xiuqin Sun
Affiliations

Isolation and Characterization of a Phosphate-Solubilizing Halophilic Bacterium Kushneria sp. YCWA18 from Daqiao Saltern on the Coast of Yellow Sea of China

Fengling Zhu et al. Evid Based Complement Alternat Med. 2011.

Abstract

Phosphate-solubilizing bacteria (PSB) function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Isolation and application of salt-tolerant or halophilic PSB will facilitate the development of saline-alkali soil-based agriculture. A moderately halophilic bacterium was isolated from the sediment of Daqiao saltern on the eastern coast of China, which also performs phosphate-solubilizing ability. The bacterium was assigned to genus Kushneria according to its 16S rRNA gene sequence, and accordingly named as Kushneria sp. YCWA18. The fastest growth was observed when the culturing temperature was 28°C and the concentration of NaCl was 6% (w/v). It was founds that the bacterium can survive at a concentration of NaCl up to 20%. At the optimum condition, the bacterium solubilized 283.16 μg/mL phosphorus in 11 days after being inoculated in 200 mL Ca(3)(PO(4))(2) containing liquid medium, and 47.52 μg/mL phosphorus in 8 days after being inoculated in 200 mL lecithin-containing liquid medium. The growth of the bacterium was concomitant with a significant decrease of acidity of the medium.

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Figures

Figure 1
Figure 1
Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences, showing the position of strain YCWA18 with respect to related species.
Figure 2
Figure 2
Test of NaCl and pH tolerance of the isolate ((a): NaCl; (b): pH).
Figure 3
Figure 3
The phosphate-solubilizing zone formed on medium containing Ca3(PO4)2 (a) and lecithin (b). Bar: 1 cm.
Figure 4
Figure 4
Phosphorus-solubilizing performance of the isolate ((a) P-solubilizing ability; (b) pH value of the medium).
Figure 5
Figure 5
The influence of temperature (a) and acidity (b) on the phosphorus solubilization in Ca3(PO4)2 containing medium.
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