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. 2011 Aug 9;23(30):3435-40.
doi: 10.1002/adma.201101516. Epub 2011 Jul 1.

Nanofibrous lipid membranes capable of functionally immobilizing antibodies and capturing specific cells

Zhengbao Zha  1 , Celine Cohn, Zhifei Dai, Weiguo Qiu, Jinhong Zhang, Xiaoyi Wu
Affiliations

Nanofibrous lipid membranes capable of functionally immobilizing antibodies and capturing specific cells

Zhengbao Zha et al. Adv Mater. .
No abstract available

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Figures

Figure 1
Figure 1
A combined sol-gel and electrospinning process for engineering nanofibrous CSS membrane. (a) The chemical structure of CSS and schematic of CSS hydrolysis and polymerization prior to electrospinning. (b) SEM images of electrospun CSS nanofibers. (c) Water contact angle images. (d) TEM images.
Figure 2
Figure 2
(a) Schematic of CSS polymerization and micelle formation. (b) SEM images of CSS fi bers that were immersed in 1× PBS for 72 h, 0.1% Triton X-100, and 1% Triton X-100 for 48 hrs. (c) A representative force-separation curve of single CSS fibers obtained via AFM nanoindentation. Inset: a typical AFM image of CSS fibers in the reflection mode.
Figure 3
Figure 3
Immunofluorescent (a) and SEM (b) images of Granta-22 cells captured on the anti-CD20 mAb functionalized CSS membranes. The cells were fixed at indicated times, and stained for nucleus with DAPI (in blue) and for actin with phalloidin (in red). Inserts show the F-actin accumulated in the lamellipodias. Control studies (c): capturing no Granta-22 cells using the non-functionalized membranes, and no Jurkat T cells using the functionalized membranes in 45 min.
Figure 4
Figure 4
The number of the captured Granta-22 cells as a function of time. The solid line represents predictions from the first-order kinetic model, while symbols with error bars represent experimental data.
See this image and copyright information in PMC

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