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. 2011 Jul 3;8(8):649-51.
doi: 10.1038/nmeth.1640.

Megapixel digital PCR

Kevin A Heyries  1 , Carolina Tropini, Michael Vaninsberghe, Callum Doolin, Oleh I Petriv, Anupam Singhal, Kaston Leung, Curtis B Hughesman, Carl L Hansen
Affiliations

Megapixel digital PCR

Kevin A Heyries et al. Nat Methods. .

Abstract

We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.

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References

    1. Hum Pathol. 2006 Nov;37(11):1458-64 - PubMed
    1. Biochemistry. 1988 Aug 9;27(16):6008-13 - PubMed
    1. Nat Methods. 2006 Feb;3(2):95-7 - PubMed
    1. Nat Methods. 2006 Jul;3(7):551-9 - PubMed
    1. Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16266-71 - PubMed

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