Survival of embryos and calves derived from somatic cell nuclear transfer in cattle: a nationwide survey in Japan

Abstract

To obtain data concerning the survival of embryos and calves derived from somatic cell nuclear transfer (SCNT) in Japan, a nationwide survey was carried out in April, 2009. As a result, data concerning 3264 embryo transfers (ETs) with SCNT embryos which produced 301 calves were accumulated and their survival was analyzed. The present survey revealed that survival rates of transferred bovine embryos and produced calves derived from SCNT had not improved over a decade (1998-2007). A remarkable feature of the pregnancies with SCNT embryos was a high incidence of spontaneous abortions. When the decade was divided by the occurrence of bovine spongiform encephalopathy (BSE) in 2001, significant decreases in the 'after BSE' period (2002-2007) were observed in the percentages of calves born (P<0.01), calves living at birth (P<0.05), calves living for 24 h (P<0.05) and 6 months (P<0.01). Abortions that occurred during 61-99 days after ETs were significantly increased (P<0.01) in the 'after BSE' period. Certain kinds of regeneration that occurred in oocytes during the 15-20 h of storage of bovine ovaries at 10-15 °C as a part of BSE inspection might have had some negative effects on SCNT embryos when these oocytes were used as recipients of SCNT.