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. 2011 Oct;66(10):2248-54.
doi: 10.1093/jac/dkr289. Epub 2011 Jul 6.

Acinetobacter baumannii isolates from pets and horses in Switzerland: molecular characterization and clinical data

Andrea Endimiani  1 , Kristine M Hujer, Andrea M Hujer, Isabelle Bertschy, Alexandra Rossano, Christoph Koch, Vinzenz Gerber, Thierry Francey, Robert A Bonomo, Vincent Perreten
Affiliations

Acinetobacter baumannii isolates from pets and horses in Switzerland: molecular characterization and clinical data

Andrea Endimiani et al. J Antimicrob Chemother. 2011 Oct.

Abstract

Objectives: We investigated whether Acinetobacter baumannii isolates of veterinary origin shared common molecular characteristics with those described in humans.

Methods: Nineteen A. baumannii isolates collected in pets and horses were analysed. Clonality was studied using repetitive extragenic palindromic PCR (rep-PCR) and multilocus sequence typing (MLST). PCR and DNA sequencing for various β-lactamase, aminoglycoside-modifying enzyme, gyrA and parC, ISAba1 and IS1133, adeR and adeS of the AdeABC efflux pump, carO porin and class 1/2/3 integron genes were performed.

Results: Two main clones [A (n = 8) and B (n = 9)] were observed by rep-PCR. MLST indicated that clone A contained isolates of sequence type (ST) ST12 (international clone II) and clone B contained isolates of ST15 (international clone I). Two isolates of ST10 and ST20 were also noted. Seventeen isolates were resistant to gentamicin, 12 to ciprofloxacin and 3 to carbapenems. Isolates of ST12 carried bla(OXA-66), bla(ADC-25), bla(TEM-1), aacC2 and IS1133. Strains of ST15 possessed bla(OXA-69), bla(ADC-11), bla(TEM-1) and a class 1 integron carrying aacC1 and aadA1. ISAba1 was found upstream of bla(ADC) (one ST10 and one ST12) and/or bla(OXA-66) (seven ST12). Twelve isolates of different STs contained the substitutions Ser83Leu in GyrA and Ser80Leu or Glu84Lys in ParC. Significant disruptions of CarO porin and overexpressed efflux pumps were not observed. The majority of infections were hospital acquired and in animals with predisposing conditions for infection.

Conclusions: STs and the molecular background of resistance observed in our collection have been frequently described in A. baumannii detected in human patients. Animals should be considered as a potential reservoir of multidrug-resistant A. baumannii.

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Figures

Figure 1.
Figure 1.
Results of rep-PCR and MLST analyses for the 19 A. baumannii isolates responsible for infection in animals (12 dogs, 4 horses and 3 cats). rep-PCR identified two main clones (A and B) that shared <60% band homology. Isolates included in clone A showed 100% band homology, whereas those included in clone B demonstrated slightly different band patterns. By using MLST, isolates included in clone A were ST12 (international clone II), whereas those in clone B were ST15 (international clone I). Two A. baumannii isolates were ST10 (a variant related to ST12) and ST20.
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