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Review
. 2011 Nov-Dec;29(6):830-9.
doi: 10.1016/j.biotechadv.2011.06.017. Epub 2011 Jun 30.

Advances in microfluidic PCR for point-of-care infectious disease diagnostics

Seungkyung Park  1 , Yi Zhang, Shin Lin, Tza-Huei Wang, Samuel Yang
Affiliations
Review

Advances in microfluidic PCR for point-of-care infectious disease diagnostics

Seungkyung Park et al. Biotechnol Adv. 2011 Nov-Dec.

Abstract

Global burdens from existing or emerging infectious diseases emphasize the need for point-of-care (POC) diagnostics to enhance timely recognition and intervention. Molecular approaches based on PCR methods have made significant inroads by improving detection time and accuracy but are still largely hampered by resource-intensive processing in centralized laboratories, thereby precluding their routine bedside- or field-use. Microfluidic technologies have enabled miniaturization of PCR processes onto a chip device with potential benefits including speed, cost, portability, throughput, and automation. In this review, we provide an overview of recent advances in microfluidic PCR technologies and discuss practical issues and perspectives related to implementing them into infectious disease diagnostics.

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Figures

Figure 1
Figure 1
Schematic illustrations of types of microfluidic PCR chip designs. a) stationary chamber system b) continuous flow system
Figure 2
Figure 2
Schematic illustrations of droplet based microfluidic PCR chips. a) Droplet emulsion system b) Open surface droplet based system
See this image and copyright information in PMC

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