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. 2011 Sep;55(9):4398-401.
doi: 10.1128/AAC.00329-11. Epub 2011 Jul 11.

First outbreak of a plasmid-mediated carbapenem-hydrolyzing OXA-48 beta-lactamase in Klebsiella pneumoniae in Spain

Cristina Pitart  1 , Mar Solé, Ignasi Roca, Anna Fàbrega, Jordi Vila, Francesc Marco
Affiliations

First outbreak of a plasmid-mediated carbapenem-hydrolyzing OXA-48 beta-lactamase in Klebsiella pneumoniae in Spain

Cristina Pitart et al. Antimicrob Agents Chemother. 2011 Sep.

Abstract

Twenty Klebsiella pneumoniae isolates producing OXA-48 were collected from April 2009 to September 2010. Strains were clonally related and coproduced a CTX-M-15 β-lactamase. A conjugative plasmid of circa 70 kb carrying bla(OXA-48) was identified. Eleven isolates showed low-level resistance to carbapenems, whereas nine showed high-level resistance. Decreased expression of OmpK36 was related to high-level resistance to carbapenems. The isolates belonged to sequence type 101 (ST101). This is the first outbreak caused by an OXA-48-producing K. pneumoniae strain in Spain.

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Figures

Fig. 1.
Fig. 1.
XbaI PFGE patterns of OXA-48-producing K. pneumoniae isolates. Lane 1, isolate 5837; lane 2, isolate 5836; lane 3, isolate 5834; lane 4, isolate 5839; lane 5, isolate 5835; lane 6, isolate 6083; lane 7, isolate 6168; lane 8, isolate 6167; lane 9, isolate 6440; lane 10, isolate 7310; lane 11, isolate 6082; lane 12, isolate 7591; lane 13, isolate 7619; lane 14, isolate 7680; lane 15, isolate 7745; lane 16, isolate 7951; lane17, isolate 8037; lane 18, isolate 8064, lane 19; isolate 8268; lane 20, isolate 7911. MWM, molecular weight marker.
Fig. 2.
Fig. 2.
SDS-PAGE of OMPs from K. pneumoniae isolates. Lane 1, CSUB 10S control strain expressing OmpK36; lane 2, CSUB 10R control strain not expressing OmpK36; lane 3, K. pneumoniae 5837; lane 4, K. pneumoniae 7680. Only the relevant parts of the gel are shown.
See this image and copyright information in PMC

References

    1. Aubert D., Naas T., Heritier C., Poirel L., Nordmann P. 2006. Functional characterization of IS1999, an IS4 family element involved in mobilization and expression of β-lactam resistance genes. J. Bacteriol. 188:6506–6514 - PMC - PubMed
    1. Bell J. M., Mathai D., Jones R. N., Turnidge J. D. 2010. Emergence of OXA-48 carbapenemases among Klebsiella spp. from India, abstr. C2-650. Abstr. 50th Intersci. Conf. Antimicrob. Agents Chemother., Boston, MA American Society for Microbiology, Washington, DC
    1. Benouda A., Touzani O., Khairallah M. T., Araj G. F., Matar G. M. 2010. First detection of oxacillinase-mediated resistance to carbapenems in Klebsiella pneumoniae from Morocco. Ann. Trop. Med. Parasitol. 104:327–330 - PubMed
    1. Bradford P. A., et al. 2004. Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 β-lactamases in New York City. Clin. Infect. Dis. 39:55–60 - PubMed
    1. Bradford P. A., et al. 1997. Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC β-lactamase, and the loss of an outer membrane protein. Antimicrob. Agents Chemother. 41:563–569 - PMC - PubMed

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