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. 2011 Sep;55(9):4398-401.
doi: 10.1128/AAC.00329-11. Epub 2011 Jul 11.

First outbreak of a plasmid-mediated carbapenem-hydrolyzing OXA-48 beta-lactamase in Klebsiella pneumoniae in Spain

Cristina Pitart  1 , Mar Solé, Ignasi Roca, Anna Fàbrega, Jordi Vila, Francesc Marco
Affiliations

First outbreak of a plasmid-mediated carbapenem-hydrolyzing OXA-48 beta-lactamase in Klebsiella pneumoniae in Spain

Cristina Pitart et al. Antimicrob Agents Chemother. 2011 Sep.

Abstract

Twenty Klebsiella pneumoniae isolates producing OXA-48 were collected from April 2009 to September 2010. Strains were clonally related and coproduced a CTX-M-15 β-lactamase. A conjugative plasmid of circa 70 kb carrying bla(OXA-48) was identified. Eleven isolates showed low-level resistance to carbapenems, whereas nine showed high-level resistance. Decreased expression of OmpK36 was related to high-level resistance to carbapenems. The isolates belonged to sequence type 101 (ST101). This is the first outbreak caused by an OXA-48-producing K. pneumoniae strain in Spain.

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Figures

Fig. 1.
Fig. 1.
XbaI PFGE patterns of OXA-48-producing K. pneumoniae isolates. Lane 1, isolate 5837; lane 2, isolate 5836; lane 3, isolate 5834; lane 4, isolate 5839; lane 5, isolate 5835; lane 6, isolate 6083; lane 7, isolate 6168; lane 8, isolate 6167; lane 9, isolate 6440; lane 10, isolate 7310; lane 11, isolate 6082; lane 12, isolate 7591; lane 13, isolate 7619; lane 14, isolate 7680; lane 15, isolate 7745; lane 16, isolate 7951; lane17, isolate 8037; lane 18, isolate 8064, lane 19; isolate 8268; lane 20, isolate 7911. MWM, molecular weight marker.
Fig. 2.
Fig. 2.
SDS-PAGE of OMPs from K. pneumoniae isolates. Lane 1, CSUB 10S control strain expressing OmpK36; lane 2, CSUB 10R control strain not expressing OmpK36; lane 3, K. pneumoniae 5837; lane 4, K. pneumoniae 7680. Only the relevant parts of the gel are shown.
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