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. 2011:2011:548219.
doi: 10.1155/2011/548219. Epub 2011 Jun 26.

C. trachomatis in female reproductive tract infections and RFLP-based genotyping: a 16-year study from a tertiary care hospital

Satpathy Gita  1 , Mittal Suneeta, Sharma Anjana, Nayak Niranjan, Mohanty Sujata, R M Pandey
Affiliations

C. trachomatis in female reproductive tract infections and RFLP-based genotyping: a 16-year study from a tertiary care hospital

Satpathy Gita et al. Infect Dis Obstet Gynecol. 2011.

Abstract

Presence of Chlamydia trachomatis in endocervix was determined in 2466 women attending a tertiary care hospital in New Delhi, India over a period of 16 years, using a monoclonal-based direct immunofluorescence assay, tissue culture isolation, and a conventional PCR assay. Chlamydia antigen could be detected in 391 out of 2466 (15.85%) of patients studied; in 27.27% women with PID, 16.74% women with cervicitis, 16.03% women with infertility, and 12.06% women with adverse pregnancy outcomes, respectively. There was a statistically significant decreasing trend in Chlamydia antigen positivity between the years 1994-1999 and 2000-2004; the apparent decline in antigen positivity between the years 2000-2004 and 2005-2010 was not statistically significant. Antigen detection assay detected equal number of positives as the PCR assay; tissue culture isolation demonstrated lower positivity. In a few representative specimens from cervicitis patients, genotyping was done using RFLP pattern analysis of C. trachomatis MOMP gene amplified by PCR assay, all of these belonged to Chlamydia trachomatis serovar E.

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Figures

Figure 1
Figure 1
Yearwise positivity rates of Chlamydial infections (based on DFA test results) amongst various disease groups.
Figure 2
Figure 2
RFLP analysis of the PCR amplified MOMP gene from clinical specimens in 3% agarose gel.
See this image and copyright information in PMC

References

    1. Taylor-Robinson D. Genital chlamydial infections: clinical aspects, diagnosis, treatment and prevention. In: Harris JRW, Foster SM, editors. Recent Advances in Sexually Transmitted Disease and AIDS. Vol. 4. London, UK: Churchil Livinigstone; 1991. pp. 219–262.
    1. Ray K. Chlamydia trachomatis & infertility. Indian Journal of Medical Research. 2006;123(6):730–734. - PubMed
    1. Schachter J. Chlamydial infections. The New England Journal of Medicine. 1978;298:428–435. - PubMed
    1. Ridgway GL, Oriel JD. Interrelationship of C. trachomatis and other pathogens in the female genital tract. Journal of Clinical Pathology. 1977;30(10):933–936. - PMC - PubMed
    1. Arya OP, Mallinson H, Goddard AD. Epidemiological and clinical correlates of chlamydial infection of the cervix. British Journal of Venereal Diseases. 1981;57(2):118–124. - PMC - PubMed

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