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. 2011;9(6):994-1006.
doi: 10.3390/md9060994. Epub 2011 Jun 9.

Bioactive cembranoids from the dongsha atoll soft coral Sarcophyton crassocaule

Wan-Yu Lin  1 , Yi Lu  1 , Jui-Hsin Su  2   3 , Zhi-Hong Wen  1 , Chang-Feng Dai  4 , Yao-Haur Kuo  5 , Jyh-Horng Sheu  1   6
Affiliations

Bioactive cembranoids from the dongsha atoll soft coral Sarcophyton crassocaule

Wan-Yu Lin et al. Mar Drugs. 2011.

Abstract

Seven new cembranoids, sarcocrassocolides F-L (1-7), have been isolated from a soft coral Sarcophyton crassocaule. Their structures were determined by extensive spectroscopic analysis. Most new compounds exhibited significant cytotoxic activity against a limited panel of cancer cell lines, and the structure-activity relationship was studied. Compounds 1-7 were found to display significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein. Compound 4 was also found to effectively reduce the level of COX-2 protein.

Keywords: Sarcophyton crassocaule; anti-inflammatory activity; cytotoxic activity; soft coral.

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Figures

Figure 1
Figure 1
1H-1H COSY and HMBC correlations for 1, 5 and 7.
Figure 2
Figure 2
Key NOESY correlations for 1 and 2.
Figure 3
Figure 3
Key NOESY correlations for 5 and 7.
Figure 4
Figure 4
Effect of compounds 1–7 on iNOS and COX-2 proteins expression of RAW264.7 macrophage cells by immunoblot analysis: (A) Immunoblots of iNOS and β-actin; (B) Immunoblots of COX-2 and β-actin. The values are mean ± SEM (n = 6). Relative intensity of the LPS alone stimulated group was taken as 100 %.* Significantly different from LPS alone stimulated group (* P < 0.05). a stimulated with LPS, b stimulated with LPS in the presence of 1–7 (10 μM).
Chart 1.
Chart 1.
Structures of Metabolites 1–7.
See this image and copyright information in PMC

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