Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Jun;2(6):463-9.
doi: 10.1007/s13238-011-1060-z. Epub 2011 Jul 12.

CCAAT/enhancer binding proteins play a role in oriLyt-dependent genome replication during MHV-68 de novo infection

Jing Qi  1 , Danyang Gong, Hongyu Deng
Affiliations

CCAAT/enhancer binding proteins play a role in oriLyt-dependent genome replication during MHV-68 de novo infection

Jing Qi et al. Protein Cell. 2011 Jun.

Abstract

Murine gammaherpesvirus 68 (MHV-68), a member of the gammaherpesvirus family, replicates robustly in permissive cell lines and is able to infect laboratory mice. MHV-68 has emerged as a model for studying the basic aspects of viral replication and host-virus interactions of its human counterparts. Herpesvirus genome replication is mediated through a cis-element in the viral genome called the origin of lytic replication (oriLyt). A family of transcription factors, CCAAT/enhancer binding proteins (C/EBPs), assists in oriLyt-mediated DNA replication during gammaherpesvirus reactivation. In this study, we examined the role of C/EBPs in gammaherpesvirus DNA replication during de novo infection, using MHV-68 as a model. We found that C/EBP α and β bind to the CCAAT boxes in the MHV-68 oriLyt core region both in vitro and in vivo, as demonstrated by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. A dominant negative form of C/EBPs significantly impaired the lytic replication efficiency of MHV-68 on both the plasmid and genome levels in a replication assay, indicating that functional C/EBPs are required for maximal MHV-68 genome DNA replication. Collectively, our data demonstrate that C/EBPs interact with the oriLyt core region and play an important role in MHV-68 lytic DNA replication during de novo infection.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Collins C.M., Medveczky M.M., Lund T., Medveczky P.G. The terminal repeats and latency-associated nuclear antigen of herpesvirus saimiri are essential for episomal persistence of the viral genome. J Gen Virol. 2002;83:2269–2278. doi: 10.1099/0022-1317-83-9-2269. - DOI - PubMed
    1. Deng H., Chu J.T., Park N.H., Sun R. Identification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68. J Virol. 2004;78:9123–9131. doi: 10.1128/JVI.78.17.9123-9131.2004. - DOI - PMC - PubMed
    1. Efstathiou S., Ho Y.M., Hall S., Styles C.J., Scott S.D., Gompels U.A. Murine herpesvirus 68 is genetically related to the gammaherpesviruses Epstein-Barr virus and herpesvirus saimiri. J Gen Virol. 1990;71:1365–1372. doi: 10.1099/0022-1317-71-6-1365. - DOI - PubMed
    1. Gong D., Qi J., Arumugaswami V., Sun R., Deng H. Identification and functional characterization of the left origin of lytic replication of murine gammaherpesvirus 68. Virology. 2009;387:285–295. doi: 10.1016/j.virol.2009.02.029. - DOI - PMC - PubMed
    1. Hu J., Renne R. Characterization of the minimal replicator of Kaposi’s sarcoma-associated herpesvirus latent origin. J Virol. 2005;79:2637–2642. doi: 10.1128/JVI.79.4.2637-2642.2005. - DOI - PMC - PubMed

Publication types

MeSH terms