Cardioprotective activity of flax lignan concentrate extracted from seeds of Linum usitatissimum in isoprenalin induced myocardial necrosis in rats

Abstract

The objective of the study was to evaluate the cardioprotective activity of flax lignan concentrate (FLC) in isoprenalin (ISO) induced cardiotoxicity in rats. Male Wistar rats (200-230 g) were divided into three groups. Group I: control, Group II: isoprenalin, Group III: FLC (500 mg/kg, p.o.) orally for 8 days and in group II and III isoprenalin 5.25 mg/kg, s.c. on day 9 and 8.5 mg/kg on day 10. On day 10 estimation of marker enzymes in serum and haemodynamic parameters were recorded. Animals were sacrificed, histology of heart was performed. Isoprenalin showed cardiotoxicity, manifested by increased levels of marker enzymes and increased heart rate. FLC treatment reversed these biochemical changes significantly compared with ISO group. The cardiotoxic effect of isoprenalin was less in FLC pretreated animals, which was confirmed in histopathological alterations. Haemodynamic, biochemical alteration and histopathological results suggest a cardioprotective protective effect of FLC in isoprenalin induced cardiotoxicity.

Keywords: Linum usitatissimum; flax lignan concentrate; flaxseed; isoprenalin; myocardial necrosis.

Figure 1

Chromatography. A: HPTLC densitogram of SDG standard; B: HPTLC densitogram of FLC; C: Calibration curves in linear in the range of 200, 400, 800, 1 600, 2 400, 3 200 ng for SDG standard; D: UV spectra of standard SDG and SDG present in the FLC samples obtained by HPTLC spot scanning from 200 to 400 nm.

Figure 2

Photomicrographs of histopathological changes of rat heart at 20× and 40×. A (20×) (-, absence of change), A1 (40×) Control group, B (20×) (+ +, 30–60% necrosis), B1 (40×) ISO (8.5 mg/kg, s. c.) group, C (20×) (+, 0–30% necrosis), C1 (40×) FLC (500 mg/kg, p.o.)+ISO (8.5 mg/kg s. c.) U - Unremarkable, NM - Necrotic myocardial cells, I - Inflammatory cells.