Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Dec 25;18(24):7389-96.
doi: 10.1093/nar/18.24.7389.

Transcription regulates oxolinic acid-induced DNA gyrase cleavage at specific sites on the E. coli chromosome

G Condemine  1 , C L Smith
Affiliations
Free PMC article

Transcription regulates oxolinic acid-induced DNA gyrase cleavage at specific sites on the E. coli chromosome

G Condemine et al. Nucleic Acids Res. .
Free PMC article

Abstract

Prominent DNA gyrase-mediated cleavage sites, induced by oxolinic acid, occur at specific, but infrequent, locations on the Escherichia coli chromosome. These sites, which we call toposites, may represent high affinity DNA gyrase binding sites or may mark chromosomal regions that accumulate superhelical stress. Toposites are usually grouped in 5 to 10 kb clusters that are mostly 50 to 100 kb apart. The total number of clusters on the chromosome is between 50 and 100. The location of sites depends on the local sequence. The extent of DNA gyrase cleavage at toposites can be strongly modulated by transcription occurring at as far as 35 kb away.

PubMed Disclaimer

References

    1. J Bacteriol. 1968 Oct;96(4):1200-7 - PubMed
    1. J Bacteriol. 1989 Feb;171(2):1214-8 - PubMed
    1. J Mol Biol. 1972 Nov 14;71(2):127-47 - PubMed
    1. Bacteriol Rev. 1972 Dec;36(4):525-57 - PubMed
    1. Nucleic Acids Res. 1976 Sep;3(9):2387-98 - PubMed

Publication types