Review
doi: 10.1007/978-1-61779-163-5_1.
Laser capture microdissection: methods and applications
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- PMID: 21761290
- DOI: 10.1007/978-1-61779-163-5_1
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Review
Laser capture microdissection: methods and applications
Methods Mol Biol.
2011.
Abstract
Laser microdissection is a nonmolecular, minimally disruptive method to obtain cytologically and/or phenotypically defined cells or groups of cells from heterogeneous tissues. It is a versatile technology and allows the preparation of homogenous isolates of specific subpopulations of cells from which RNA/DNA or protein can be extracted for RT-polymerase chain reaction (PCR), quantitative PCR, Western blot analyses, and mass spectrophotometry.
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