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Review
. 2011:755:1-15.
doi: 10.1007/978-1-61779-163-5_1.

Laser capture microdissection: methods and applications

Kristen Decarlo  1 , Andrew Emley, Ophelia E Dadzie, Meera Mahalingam
Affiliations
Review

Laser capture microdissection: methods and applications

Kristen Decarlo et al. Methods Mol Biol. 2011.

Abstract

Laser microdissection is a nonmolecular, minimally disruptive method to obtain cytologically and/or phenotypically defined cells or groups of cells from heterogeneous tissues. It is a versatile technology and allows the preparation of homogenous isolates of specific subpopulations of cells from which RNA/DNA or protein can be extracted for RT-polymerase chain reaction (PCR), quantitative PCR, Western blot analyses, and mass spectrophotometry.

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