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. 2011 Sep;71(1):1-5.
doi: 10.1016/j.diagmicrobio.2011.04.014. Epub 2011 Jul 16.

Identification of Aeromonas isolates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry

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Identification of Aeromonas isolates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry

Brigitte Lamy et al. Diagn Microbiol Infect Dis. 2011 Sep.

Abstract

We evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying aeromonads with an extraction procedure. Genus-level accuracy was 100%. Compared to rpoB gene sequencing, species-level accuracy was 90.6% (29/32) for type and reference strains and 91.4% for a collection of 139 clinical and environmental isolates, making this system one of the most accurate and rapid methods for phenotypic identification. The reliability of this technique was very promising, although some improvements in database composition, taxonomy, and discriminatory power are needed.

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