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. 1990 Sep;56(2):237-42.
doi: 10.1016/0022-2011(90)90106-g.

An in vitro system for testing Bacillus thuringiensis toxins: the lawn assay

J L Gringorten  1 , D P Witt, R E Milne, P G Fast, S S Sohi, K van Frankenhuyzen
Affiliations

An in vitro system for testing Bacillus thuringiensis toxins: the lawn assay

J L Gringorten et al. J Invertebr Pathol. 1990 Sep.

Abstract

A cell assay system was developed that allows Bacillus thuringiensis delta-endotoxins activated at high pH (10.5) to be tested in vitro without causing alkaline injury to target cells. The assay is carried out on a lawn of gel-suspended cells, requires only 1 microliter of sample per dose, and is quantitative, rapid, and sensitive. The threshold dose for toxicity of B. thuringiensis subsp. kurstaki HD-73 with IPRI-CF-1 cells was 24 pg protein. The assay is also very useful for identifying antibodies which inhibit toxicity and for detecting beta-exotoxin.

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