The In Vitro Effects of Aqueous and Ethanolic Extracts of the Leaves of Ageratum conyzoides (Asteraceae) on Three Life Cycle Stages of the Parasitic Nematode Heligmosomoides bakeri (Nematoda: Heligmosomatidae)

Abstract

A comparative in vitro study was carried out to determine the ovicidal and larvicidal activity of aqueous and ethanolic extracts of Ageratum conyzoides (Asteraceae) leaves on the eggs (unembryonated and embryonated), first and second larval stages of Heligmosomoides bakeri. Four different concentrations (0.625, 1.25, 2.5, and 3.75 mg·mL(-1)) of both aqueous and ethanolic extracts were tested. Distilled water and 5% tween were used as negative controls in the bioassay. In fact, they did not affect development of eggs, hatching, and larval survival. The extract activities were dose dependent. The ethanolic extract was more potent against embryonation (39.6 ± 2.9%) than the aqueous extract (53.3 ± 10.9%) at the highest concentration (3.75 mg·ml(-1)). Both types of extracts killed larvae. Mebendazole proved more lethal (EC(50) of 0.745 and 0.323 mg·mL(-1), resp., for L(1) and L(2) larvae). The aqueous extracts were the least lethal (EC(50) of 4.76 and 2.29 mg·mL(-1), resp., for L(1) and L(2) larvae). The ethanolic extracts showed intermediate activity (EC(50) of 1.323 and 1.511 mg·mL(-1), resp., for L(1) and L(2) larvae). It is concluded that the ovicidal and larvicidal properties of aqueous and ethanolic extracts of Ageratum conyzoides leaves are demonstrated in this work.