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. 2011 Sep 19;50(39):9058-61.
doi: 10.1002/anie.201102776. Epub 2011 Jul 19.

Label-free detection of single-base mismatches in DNA by surface-enhanced Raman spectroscopy

Evanthia Papadopoulou  1 , Steven E J Bell
Affiliations
Free PMC article

Label-free detection of single-base mismatches in DNA by surface-enhanced Raman spectroscopy

Evanthia Papadopoulou et al. Angew Chem Int Ed Engl. .
Free PMC article
No abstract available

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Figures

Figure 1
Figure 1
SERS spectra of 10−5 m solutions of a) thermally treated thiolated HS-DNA-1, b) untreated, thiolated HS-DNA-1, and c) unthiolated DNA-1. All spectra were recorded on citrate-reduced Ag colloids and aggregated with 0.1 m MgSO4.
Figure 2
Figure 2
SERS spectra of 10−6 m solutions of the 24-mers a) poly A, b) 10−5 dGMP, c) poly C, and d) poly T on hydroxylamine-reduced Ag colloid aggregated with 0.1 m MgSO4.
Figure 3
Figure 3
SERS spectra of a) DNA-2 and b) DNA-3. c) Simple difference spectrum of DNA-3 minus DNA-2 ((b)−(a)) shown with its intensity expanded three times. d) Model difference spectrum, dGMP minus poly A. All spectra are for hydroxylamine-reduced Ag colloids aggregated with 0.1 m MgSO4.
Figure 4
Figure 4
SERS spectra of a) DNA-4 and b) DNA-5. c) Simple difference spectrum of DNA-5 minus DNA-4 ((b)−(a)) shown with its intensity expanded eight times. d) Model difference spectrum, poly A minus poly C. All spectra are for hydroxylamine-reduced Ag colloids aggregated with 0.1 m MgSO4.
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