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Comparative Study
. 2011 Sep;49(9):3365-7.
doi: 10.1128/JCM.02231-10. Epub 2011 Jul 20.

Improved detection of respiratory viruses in pediatric outpatients with acute respiratory illness by real-time PCR using nasopharyngeal flocked swabs

Patrick Kiio Munywoki  1 , Fauzat Hamid, Martin Mutunga, Steve Welch, Patricia Cane, D James Nokes
Affiliations
Comparative Study

Improved detection of respiratory viruses in pediatric outpatients with acute respiratory illness by real-time PCR using nasopharyngeal flocked swabs

Patrick Kiio Munywoki et al. J Clin Microbiol. 2011 Sep.

Abstract

Detection of respiratory viruses by real-time multiplexed PCR (M-PCR) and of respiratory syncytial virus (RSV) by M-PCR and immunofluorescence (IF) was evaluated using specimens collected by nasopharyngeal flocked swabbing (NFS) and nasal washes (NW). In children with mild respiratory illness, NFS collection was superior to NW collection for detection of viruses by M-PCR (sensitivity, 89.6% versus 79.2%; P = 0.0043). NFS collection was noninferior to NW collection in the detection of RSV by IF.

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References

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