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. 2010;2(3):e2010032.
doi: 10.4084/MJHID.2010.032. Epub 2010 Nov 15.

Decreased CD10 Expression in the Bone Marrow Neutrophils of HIV Positive Patients

Annemarie van de Vyver  1 , Eluned Delport, Adele Visser
Affiliations

Decreased CD10 Expression in the Bone Marrow Neutrophils of HIV Positive Patients

Annemarie van de Vyver et al. Mediterr J Hematol Infect Dis. 2010.

Abstract

Background: HIV-1 infection is associated with various quantitative and qualitative changes in haemopoietic cells. Clear distinction between primary myelodysplastic syndrome (MDS) and secondary dysplasia may not always be possible. Adjunctive analyses used in the diagnosis of MDS include cytogenetics and flow cytometry (FCM). Much focus has been placed on establishing FCM guidelines aiding in the diagnosis of MDS, and to distinguish this condition from secondary dysplastic changes. One of the parameters often cited is the CD10 expression on the granulocyte population, as this marker denotes granulocytic maturation.

Aims: To determine the expression level of CD10 on granulocytes in HIV positive patients.

Methods: In total, 117 HIV-1 positive and 29 HIV-1 negative patients were included in this study. Bone marrow aspirate samples were evaluated in terms of morphological abnormality as well as CD10 expression on the granulocytic population.

Results: The average CD10 expression among the HIV-1 positive patients were markedly reduced, at 18.4%, and 113 patients (96.6%) of these patients had expression levels below 50%.

Discussion: Disease conditions causing secondary dysplasia, especially HIV-1 infection, is associated with a marked reduction in CD10 expression on the granulocyte population independent from the presence of myelodysplastic features. This marker is therefore of doubtful significance as a diagnostic tool in distinguishing between primary and secondary dysplasia.

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Figures

Figure 1:
Figure 1:
Granulocytes defined based on a. Side scatter (SS) versus Forward Scatter (FS) (population A) and b. CD45 versus SS (population B).
Figure 2:
Figure 2:
CD10 expression evaluated by a. CD5 FITC versus CD10-PE and b. CD10-PE versus CD19-ECD. Both sets were gated on population A as defined in figure 1, identified as granulocytes. Two discrete populations can be demonstrated in each defined as either CD10 positive (population C and I) or negative (populations D and H).
Figure 3.
Figure 3.
CD10 expression depicted as a Box and Whisker plot
See this image and copyright information in PMC

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References

    1. Alexaki A, Wigdahl B. “HIV-1 infection of bone marrow hematopoietic progenitor cells and their role in trafficking and viral dissemination.”. PLoS Path. 2008;4(12):1–12. - PMC - PubMed
    1. Baurmann H, Schwarz T, et al. “Acute parvovirus B19 infection mimicking myelodysplatic syndrome of the bone marrow.”. Ann Hematol. 1992;64:43–45. - PubMed
    1. Bowen D, Culligan D, et al. “Guidelines for the diagnosis and therapy of adult myelodysplastic sydnromes.”. Br J Haematol. 2003;120:187–200. - PubMed
    1. Cherian S, Moore J, et al. “Peripheral Blood MDS Score: A New Flow Cytometric Tool for the Diagnosis of Myelodysplastic Syndromes.”. Cytometry Part B. 2005;64B:9–17. - PubMed
    1. Claster S. “Biology of Anaemia: Differential Diagnosis and Treatment Options in Human Immunodeficiency Virus Infection.”. J Infect Dis. 2002;185(Suppl 2):S105–109. - PubMed