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. 2011 Jul;31(3):212-8.
doi: 10.3343/kjlm.2011.31.3.212. Epub 2011 Jun 28.

Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells

Hee Soon Cho  1 , In Hwan Song, So-Young Park, Min Cheol Sung, Myun-Whan Ahn, Kyung Eun Song
Affiliations

Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells

Hee Soon Cho et al. Korean J Lab Med. 2011 Jul.

Abstract

Background: The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal stem cells (HMSCs).

Methods: The concentrations of 7 representative GFs in activated PRP (aPRP) were measured using ELISA. The effects of PRP on the proliferation and alkaline phosphatase (ALP) activity of HMSCs were examined using several concentrations of aPRP from 3 donors; the relationships between the GF levels and these biologic effects were then evaluated using 10% aPRP from 5 subgroups derived from 39 total donors. HMSCs were cultured in DMEM with the addition of aPRP for 4 or 12 days; then, DNA content and ALP activity were measured.

Results: The quantity of DNA increased significantly at a 10% concentration of aPRP, but the ALP activity was suppressed at this concentration of aPRP. The GF concentrations varied among donors, and 5 subgroups of characteristic GF release patterns were identified via cluster analysis. DNA levels differed significantly between groups and tended to be higher in groups with higher concentrations of transforming growth factor-beta1 (TGF-β1) and platelet-derived growth factors (PDGFs). DNA quantity was positively correlated with TGF-β1 concentration, and was negatively correlated with donor age. ALP activity was negatively correlated with PDGF-BB concentration.

Conclusions: The varying GF concentrations may result in different biologic effects; thus, individual differences in GF levels should be considered for reliable interpretation of the biologic functions and standardized application of PRP.

Keywords: Growth factors; Mesenchymal stem cells; Platelet-derived growth factor-BB; Platelet-rich plasma; Transforming growth factor-β1.

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Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1
The DNA concentration (A) and ALP activity (B) of human mesenchymal stem cells after the addition of 1%, 3%, 10%, and 30% activated PRP or activated PPP. Each value is the mean of 3 donors, with the error bar showing one standard deviation (*P<0.05; **P<0.01 compared with control). Abbreviations: ALP, alkaline phosphatase; PRP, platelet-rich plasma; PPP, platelet-poor plasma.
Fig. 2
Fig. 2
Quantification of growth factors in activated PRP and activated PPP (P<0.05 between PRP and PPP, except for IGF-1). Abbreviations: TGF-β1, transforming growth factor-β1; PDGF, platelet-derived growth factor; IGF-1, insulin-like growth factor-1; FGF-b, fibroblast growth factor-basic; VEGF, vascular endothelial growth factor.
See this image and copyright information in PMC

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