Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011:2011:587604.
doi: 10.1155/2011/587604. Epub 2011 Jun 7.

Poly(amidoamine)-Cholesterol Conjugate Nanoparticles Obtained by Electrospraying as Novel Tamoxifen Delivery System

R Cavalli  1 , A Bisazza, R Bussano, M Trotta, A Civra, D Lembo, E Ranucci, P Ferruti
Affiliations

Poly(amidoamine)-Cholesterol Conjugate Nanoparticles Obtained by Electrospraying as Novel Tamoxifen Delivery System

R Cavalli et al. J Drug Deliv. 2011.

Abstract

A new poly(amidoamine)-cholesterol (PAA-cholesterol) conjugate was synthesized, characterized and used to produce nanoparticles by the electrospraying technique. The electrospraying is a method of liquid atomization that consists in the dispersion of a solution into small charged droplets by an electric field. Tuning the electrospraying process parameters spherical PAA-chol nanoparticles formed. The PAA-cholesterol nanoparticles showed sizes lower than 500 nm and spherical shape. The drug incorporation capacity was investigated using tamoxifen, a lipophilic anticancer drug, as model drug. The incorporation of the tamoxifen did not affect the shape and sizes of nanoparticles showing a drug loading of 40%. Tamoxifen-loaded nanoparticles exhibited a higher dose-dependent cytotoxicity than free tamoxifen, while blank nanoparticles did not show any cytotoxic effect at the same concentrations. The electrospray technique might be proposed to produce tamoxifen-loaded PAA-chol nanoparticle in powder form without any excipient in a single step.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Scheme of the apparatus for electrospraying.
Figure 2
Figure 2
Chemical structure of the PAA-cholesterol conjugate.
Figure 3
Figure 3
Surface tension of PAA-cholesterol conjugate in water.
Figure 4
Figure 4
SEM image of blank PAA-cholesterol nanoparticles.
Figure 5
Figure 5
Fluorescent PAA-cholesterol nanoparticles containing Rhodamine B (fluorescent microscopy).
Figure 6
Figure 6
Internalization of fluorescent PAA-cholesterol nanoparticles on Vero cells analysed by confocal laser scanning microscopy.
Figure 7
Figure 7
SEM image of In vitro release of the formulations.
Figure 8
Figure 8
Thermal analysis of tamoxifen and tamoxifen-loaded nanoparticles.
Figure 9
Figure 9
In vitro release of tamoxifen from the formulations.
Figure 10
Figure 10
Cell viability of MCF-7 breast cancer cells incubated for 24, 48, and 72 h (a, b, and c panels, resp.) with free tamoxifen (Tamoxifen), tamoxifen-loaded nanoparticles (tamoxifen-NP), empty nanoparticles (NP), or DMSO. Each bar represents the mean of three samples ± SD.
See this image and copyright information in PMC

References

    1. Cismaru L, Popa M. Polymeric nanoparticles with biomedical applications. Revue Roumaine de Chimie. 2010;55(8):433–442.
    1. Chan JM, Valencia PM, Zhang L, Langer R, Farokhzad OC. Polymeric nanoparticles for drug delivery. Methods in Molecular Biology. 2010;624:163–175. - PubMed
    1. Chauvierre C, Vauthier C, Labarre D, Couvreur P, Marden MC, Leclerc L. A new generation of polymer nanoparticles for drug delivery. Cellular and Molecular Biology. 2004;50(3):233–239. - PubMed
    1. Singh R, Singh S, Lillard JW. Past, present, and future technologies for oral delivery of therapeutic proteins. Journal of Pharmaceutical Sciences. 2008;97(7):2497–2523. - PMC - PubMed
    1. Jayasinghe SN, Sullivan AC. Electrohydrodynamic atomization: an approach to growing continuous self-supporting polymeric fibers. Journal of Physical Chemistry B. 2006;110(6):2522–2528. - PubMed

LinkOut - more resources