Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1990 Feb;97(2):83-5.

A competition enzyme immunoassay for brucellosis diagnosis

A P MacMillan  1 , I Greiser-Wilke, V Moennig, L A Mathias
Affiliations
  • PMID: 2178906
Comparative Study

A competition enzyme immunoassay for brucellosis diagnosis

A P MacMillan et al. Dtsch Tierarztl Wochenschr. 1990 Feb.

Abstract

Two monoclonal antibodies (MAbs) conjugated with horseradish peroxidase were used independently in a competitive enzyme immunoassay (cEIA) to detect Brucella specific antibodies in 1120 sera from Brucella-free cattle, 61 from cattle known to be infected with B. abortus, and 207 sera from vaccinated calves. The results were compared to those obtained in the complement fixation test (CFT). The cEIA with both MAbs proved to be more sensitive than the CFT because no false-negative results were obtained. In addition, discrimination between sera from infected and vaccinated animals was more evident.

PubMed Disclaimer

Publication types

Substances