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Comment
. 2011 Jul 29;18(7):821-3.
doi: 10.1016/j.chembiol.2011.07.001.

Finding the missing code of RNA recognition by PUF proteins

Yu Chen  1 , Gabriele Varani
Affiliations
Comment

Finding the missing code of RNA recognition by PUF proteins

Yu Chen et al. Chem Biol. .

Abstract

Pumilio and FBF homology (PUF) proteins represent highly promising candidates for engineering sequence-specific RNA recognition, but were only known to recognize G, A, and U, significantly limiting applications. Two groups (Filipovska et al., 2011; Dong et al., 2011) have now reported the discovery of the cytosine-recognition code for PUF proteins.

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Figures

FIGURE1
FIGURE1
RNA recognition by PUF proteins. Shown here is the crystal structure of an engineered PUF domain in complex with target RNA containing cytosine (2YJY.pdb; Dong et al., 2011). On the left, the RNA recognition code of PUF repeats is illustrated.
See this image and copyright information in PMC

Comment on

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