Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Mar;56(3):808-11.
doi: 10.1128/aem.56.3.808-811.1990.

Monoclonal antibody to type F Clostridium botulinum toxin

J L Ferreira  1 , M K Hamdy, S G McCay, F A Zapatka
Affiliations

Monoclonal antibody to type F Clostridium botulinum toxin

J L Ferreira et al. Appl Environ Microbiol. 1990 Mar.

Abstract

Hybridomas synthesizing monoclonal antibodies (MAbs) against type F Clostridium botulinum toxin were developed. MAb from one stable hybridoma, hybridoma 223, consisted of kappa light chains and an immunoglobulin G subclass 2a heavy chain. This MAb was used in a double-sandwich enzyme-linked immunosorbent assay to detect type F toxin in foods, culture fluids, and purified toxin preparations. The sensitivity of the double-sandwich enzyme-linked immunosorbent assay was approximately 10 mouse lethal doses of toxin per ml of toxic fluid.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Appl Microbiol. 1968 Jan;16(1):21-4 - PubMed
    1. Appl Microbiol. 1973 Apr;25(4):545-9 - PubMed
    1. Appl Microbiol. 1975 May;29(5):598-603 - PubMed
    1. Methods Enzymol. 1982;84:223-38 - PubMed
    1. Acta Pathol Microbiol Scand. 1960;48:80 - PubMed

LinkOut - more resources