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. 1990 Mar;56(3):808-11.
doi: 10.1128/aem.56.3.808-811.1990.

Monoclonal antibody to type F Clostridium botulinum toxin

J L Ferreira  1 , M K Hamdy, S G McCay, F A Zapatka
Affiliations

Monoclonal antibody to type F Clostridium botulinum toxin

J L Ferreira et al. Appl Environ Microbiol. 1990 Mar.

Abstract

Hybridomas synthesizing monoclonal antibodies (MAbs) against type F Clostridium botulinum toxin were developed. MAb from one stable hybridoma, hybridoma 223, consisted of kappa light chains and an immunoglobulin G subclass 2a heavy chain. This MAb was used in a double-sandwich enzyme-linked immunosorbent assay to detect type F toxin in foods, culture fluids, and purified toxin preparations. The sensitivity of the double-sandwich enzyme-linked immunosorbent assay was approximately 10 mouse lethal doses of toxin per ml of toxic fluid.

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References

    1. Appl Microbiol. 1968 Jan;16(1):21-4 - PubMed
    1. Appl Microbiol. 1973 Apr;25(4):545-9 - PubMed
    1. Appl Microbiol. 1975 May;29(5):598-603 - PubMed
    1. Methods Enzymol. 1982;84:223-38 - PubMed
    1. Acta Pathol Microbiol Scand. 1960;48:80 - PubMed

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