Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Apr;10(4):1822-7.
doi: 10.1128/mcb.10.4.1822-1827.1990.

Isolation of recessive (mediator-) revertants from NIH 3T3 cells transformed with a c-H-ras oncogene

H Yamada  1 , T Omata-Yamada, N Wakabayashi-Ito, S G Carter, P Lengyel
Affiliations

Isolation of recessive (mediator-) revertants from NIH 3T3 cells transformed with a c-H-ras oncogene

H Yamada et al. Mol Cell Biol. 1990 Apr.

Abstract

We have generated two serum- and anchorage-dependent revertants from NIH 3T3 cells transformed with multiple copies of the human c-H-ras oncogene. In both revertants, the c-H-ras oncogene was fully expressed. Fusion of either revertant with untransformed cells or of the two revertants with one another resulted in transformed progeny. These results indicated that the two revertants were recessive and in different complementation groups. We believe that in our two revertants some of the genes mediating the transforming activity of the c-H-ras oncogene are defective; we are attempting to identify these mediator genes.

PubMed Disclaimer

References

    1. Methods Cell Biol. 1974;8(0):75-92 - PubMed
    1. Mol Cell Biol. 1988 Jun;8(6):2419-27 - PubMed
    1. Somatic Cell Genet. 1976 Mar;2(2):165-76 - PubMed
    1. Nature. 1981 Aug 13;292(5824):595-600 - PubMed
    1. Anal Biochem. 1981 Apr;112(2):195-203 - PubMed

Publication types