Integration and transcription of mouse mammary tumor virus DNA in rat hepatoma cells
- PMID: 218215
- PMCID: PMC383010
- DOI: 10.1073/pnas.76.2.665
Integration and transcription of mouse mammary tumor virus DNA in rat hepatoma cells
Abstract
Rat hepatoma cells infected with mouse mammary tumor virus (MMTV) acquire viral DNA that becomes covalently linked to the cell DNA. Using restriction endonucleases and the DNA transfer procedure of Southern [Southern , E.M. (1975) J. Mol. Biol. 98, 503--517], we have studied the sites in cellular DNA into which MMTV DNA inserts. These experiments indicate that: (i) there are many sites in cell DNA into which MMTV DNA integrates; (ii) the junctions between viral and cellular DNA occur within a limited portion of the viral genome, (iii) clones that contain MMTV DNA do not necessarily produce viral RNA; and (iv) the extent of transcription and glucocorticoid responsiveness of MMTV proviruses may be dependent on the site(s) in cell DNA in which the viral DNA resides.
Similar articles
-
A rare common integration site of proviruses of the mouse mammary tumor virus in P-type mammary tumors of mouse strain GR.Virology. 1987 Feb;156(2):229-37. doi: 10.1016/0042-6822(87)90402-8. Virology. 1987. PMID: 3027974
-
Mapping of linear and circular forms of mouse mammary tumor virus DNA with restriction endonucleases: evidence for a large specific deletion occurring at high frequency during circularization.Proc Natl Acad Sci U S A. 1978 May;75(5):2112-6. doi: 10.1073/pnas.75.5.2112. Proc Natl Acad Sci U S A. 1978. PMID: 209450 Free PMC article.
-
Mammary tumor virus proviral DNA in normal murine tissue and non-virally induced mammary tumors.J Virol. 1981 Aug;39(2):367-76. doi: 10.1128/JVI.39.2.367-376.1981. J Virol. 1981. PMID: 6268828 Free PMC article.
-
Glucocorticoid regulation of mouse mammary tumor virus gene expression.Biochim Biophys Acta. 1979 Dec 19;560(4):487-508. doi: 10.1016/0304-419x(79)90014-3. Biochim Biophys Acta. 1979. PMID: 229906 Review.
-
Mouse mammary tumor virus: transcriptional control and involvement in tumorigenesis.Adv Cancer Res. 1984;41:155-84. doi: 10.1016/s0065-230x(08)60016-0. Adv Cancer Res. 1984. PMID: 6328901 Review. No abstract available.
Cited by
-
Molecular cloning, characterization, and genetic mapping of an endogenous murine mammary tumor virus proviral unit I of C3H/He mice.J Virol. 1985 May;54(2):285-94. doi: 10.1128/JVI.54.2.285-294.1985. J Virol. 1985. PMID: 2985797 Free PMC article.
-
Sequence organization and molecular cloning of mouse mammary tumor virus DNA endogenous to C57BL/6 mice.J Virol. 1985 May;54(2):525-31. doi: 10.1128/JVI.54.2.525-531.1985. J Virol. 1985. PMID: 2985815 Free PMC article.
-
A small region of the mouse mammary tumor virus long terminal repeat confers glucocorticoid hormone regulation on a linked heterologous gene.Proc Natl Acad Sci U S A. 1983 Oct;80(19):5866-70. doi: 10.1073/pnas.80.19.5866. Proc Natl Acad Sci U S A. 1983. PMID: 6310597 Free PMC article.
-
Glucocorticoid regulation of transcription at an amplified, episomal promoter.Mol Cell Biol. 1983 Nov;3(11):2045-57. doi: 10.1128/mcb.3.11.2045-2057.1983. Mol Cell Biol. 1983. PMID: 6318079 Free PMC article.
-
Introduction, stable integration, and controlled expression of a chimeric adenovirus gene whose product is toxic to the recipient human cell.Mol Cell Biol. 1984 Jul;4(7):1354-62. doi: 10.1128/mcb.4.7.1354-1362.1984. Mol Cell Biol. 1984. PMID: 6542172 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
