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Comment
. 2011 Aug 5;29(8):697-8.
doi: 10.1038/nbt.1934.

Targeted gene disruption in somatic zebrafish cells using engineered TALENs

Jeffry D Sander, Lindsay Cade, Cyd Khayter, Deepak Reyon, Randall T Peterson, J Keith Joung, Jing-Ruey J Yeh
Comment

Targeted gene disruption in somatic zebrafish cells using engineered TALENs

Jeffry D Sander et al. Nat Biotechnol. .
No abstract available

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Figures

Figure 1
Figure 1. Target sequences, frequencies of mutations, and sequences of mutations induced by TALE nucleases in embryonic zebrafish cells
For each pair of TALE nucleases, the wild-type (WT) target sequence is shown at the top with the intended target sites of the TALE nucleases marked in yellow. Deletions are indicated by gray highlighted red dashes and insertions by blue highlighted lower case blue letters. The sizes of the insertions (+) or deletions (Δ) are indicated to the right of each mutant allele. The number of times that each mutant allele was isolated is shown in brackets. Mutation frequencies are calculated as the number of mutant alleles isolated/the total number of alleles analyzed. For the hey2 gene, we also identified two larger deletions 142 and 303 bps in length that extend substantially beyond the intended target sites of the TALE nucleases.
See this image and copyright information in PMC

Comment on

  • A TALE nuclease architecture for efficient genome editing.
    Miller JC, Tan S, Qiao G, Barlow KA, Wang J, Xia DF, Meng X, Paschon DE, Leung E, Hinkley SJ, Dulay GP, Hua KL, Ankoudinova I, Cost GJ, Urnov FD, Zhang HS, Holmes MC, Zhang L, Gregory PD, Rebar EJ. Miller JC, et al. Nat Biotechnol. 2011 Feb;29(2):143-8. doi: 10.1038/nbt.1755. Epub 2010 Dec 22. Nat Biotechnol. 2011. PMID: 21179091

References

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    1. Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA. Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol. 2008;26:695–701. - PMC - PubMed

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