Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2011 Aug 5;29(8):715-6.
doi: 10.1038/nbt.1941.

An infrared fluorescent protein for deeper imaging

Jérôme Lecoq, Mark J Schnitzer
Comment

An infrared fluorescent protein for deeper imaging

Jérôme Lecoq et al. Nat Biotechnol. .

Abstract

A newly engineered infrared fluorescent protein will allow microscopists to peer more deeply into living animals.

PubMed Disclaimer

Conflict of interest statement

COMPETING FINANCIAL INTERESTS

The authors declare no competing financial interests.

Figures

Figure 1
Figure 1
Absorption and scattering lengths are longer for near-infrared compared with visible light. Fluorescence emission peaks for an assortment of fluorescent proteins are shown in the top panel atop plots of the absorption lengths of deoxy-hemoglobin (Hb; blue curve, left axis), oxy-hemoglobin (HbO; red curve, left axis) and water (green curve, right axis). (Note that emission peaks do not necessarily convey a fluorophore’s perceived color.) The absorption lengths for hemoglobin were calculated from the molar extinction coefficients assuming a concentration of 2.2 mM hemoglobin, which is representative of human and mouse blood. The bottom panel shows the wavelength dependence of the scattering attenuation length of a tissue sample of human skin (black trace).
See this image and copyright information in PMC

Comment on

References

    1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. Science. 1994;263:802–805. - PubMed
    1. Shaner NC, Steinbach PA, Tsien RY. Nat Methods. 2005;2:905–909. - PubMed
    1. Filonov GS, et al. Nat Biotechnol. 2011;29:757–761. - PMC - PubMed
    1. Shu X, et al. Science. 2009;324:804–807. - PMC - PubMed
    1. Helmchen F, Denk W. Nat Methods. 2005;2:932–940. - PubMed