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. 1979 Feb;76(2):949-53.
doi: 10.1073/pnas.76.2.949.

Transfection of human lymphoblastoid cells with herpes simplex viral DNA

G Miller, P Wertheim, G Wilson, J Robinson, J L Geelen, J van der Noordaa, A J van der Eb

Transfection of human lymphoblastoid cells with herpes simplex viral DNA

G Miller et al. Proc Natl Acad Sci U S A. 1979 Feb.

Abstract

The "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 10(5) cells exposed to 1 microgram of DNA, 0.5--5 cells formed an infectious center. The minimal infective dose of DNA was approximately 500 ng.

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References

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