doi: 10.1073/pnas.76.2.949.
Transfection of human lymphoblastoid cells with herpes simplex viral DNA
- PMID: 218224
- PMCID: PMC383102
- DOI: 10.1073/pnas.76.2.949
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Transfection of human lymphoblastoid cells with herpes simplex viral DNA
Proc Natl Acad Sci U S A.
1979 Feb.
Abstract
The "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 10(5) cells exposed to 1 microgram of DNA, 0.5--5 cells formed an infectious center. The minimal infective dose of DNA was approximately 500 ng.
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