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. 1990 Jan;220(2):186-90.
doi: 10.1007/BF00260480.

Molecular analysis of the mitochondrial transcription factor mtf2 of Saccharomyces cerevisiae

T Lisowsky  1
Affiliations

Molecular analysis of the mitochondrial transcription factor mtf2 of Saccharomyces cerevisiae

T Lisowsky. Mol Gen Genet. 1990 Jan.

Abstract

The nuclear gene for a new mitochondrial transcription factor (mtf2) was isolated by transformation of mutant pet-ts3504. It was localized on a 6.4 kb fragment of yeast genomic DNA by subcloning and complementation tests. Sequencing of a 1.7 kb DNA fragment revealed an open reading frame of 1320 bp. A transcript of 1400 nucleotides can be assigned to this region. Gene disruption of this reading frame in a wild-type yeast strain created a stable pet phenotype. Further analysis of this insertion mutation showed that it is allelic to the mutated gene of pet-ts3504. Comparison of the 5' upstream regions of MTF2 and a previously characterized mitochondrial transcription factor (MTF1) revealed common sequence motifs which may be important for coordinated regulation of gene expression.

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References

    1. J Biol Chem. 1986 Aug 5;261(22):10348-51 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 May;60(1):160-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 - PubMed
    1. Cell. 1980 Mar;19(3):753-64 - PubMed

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