Fabrication and characterization of gold-TiO2 core-shell nanowire for immobilization of alkaline protease

Abstract

In this work, we report enhancement of the stability and biocatalytic activities of the alkaline protease enzyme immobilized on a gold/TiO2 core-shell nanowires. Gold/TiO2 core-shell nanowires were firstly prepared by the sol-gel method using a mixture of aqueous solution of titanate and chloroauric acid (HAuCI4) under ambient temperature and atmospheric condition and calcined then. As prepared nanowire was found to have a good surface biocompatibility for immobilization of the alkaline protease enzyme. The results obtained in this work showed that alkaline protease immobilized on gold-TiO2 core shell is stable and bioactive at the alkaline pH and ambient temperature. The stability and the biocatalytic activity of the immobilized gold-TiO2 core shell nano composites were monitored by UV-visible spectroscopy. Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and dispersive analysis of X-ray (EDAX) were used to characterize the size and morphology of the prepared materials.