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. 2011 Oct;49(10):3694-6.
doi: 10.1128/JCM.01187-11. Epub 2011 Aug 10.

Evaluation of two magnetic-bead-based viral nucleic acid purification kits and three real-time reverse transcription-PCR reagent systems in two TaqMan assays for equine arteritis virus detection in semen

Fabien Miszczak  1 , Kathleen M Shuck, Zhengchun Lu, Yun Young Go, Jianqiang Zhang, Stephen Sells, Astrid Vabret, Stéphane Pronost, Guillaume Fortier, Peter J Timoney, Udeni B R Balasuriya
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Evaluation of two magnetic-bead-based viral nucleic acid purification kits and three real-time reverse transcription-PCR reagent systems in two TaqMan assays for equine arteritis virus detection in semen

Fabien Miszczak et al. J Clin Microbiol. 2011 Oct.

Abstract

This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.

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Figures

Fig. 1.
Fig. 1.
Schematic of EAV detection results for 409 equine semen samples tested by VI in high-passage RK-13 cells and by rRT-PCR using various test combinations (C1, C2, C5, and C6).
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