Phylogenetic exploration of hantaviruses in Paraguay reveals reassortment and host switching in South America

Abstract

Background: Longitudinal mark-recapture studies of rodents in two sites in the Mbaracayú Biosphere Reserve in the Interior Atlantic Forest of eastern Paraguay have revealed a complex and intriguing pattern of hantaviruses harbored by rodents in this area. Full-length sequencing and phylogenetic analyses were conducted for several rodents from Akodon montensis and Oligoryzomys fornesi. The phylogenetic relationships of these viruses were analyzed in the context of hantaviruses in South America with published S- and M-segment sequences.

Findings: Phylogenetic analyses of hantaviruses identified in the Mbaracayú Biosphere Reserve in Paraguay revealed Jabora and Juquitiba viruses are harbored by Akodon montensis and Oligoryzomys fornesi, respectively. These analyses revealed that in general the constituents of the major subclade for the S- and M-segments differ for the South American hantaviruses. Further, the two major groups within subclade C for the M-segment reflect in general the lethality associated with the viruses within each group.

Conclusions: Phylogenetic studies of Jabora and Juquitiba viruses and other Paraguayan viruses in the context of American hantaviruses revealed reassortment and host-switching in the evolution of South American hantaviruses.

Figure 1

Bayesian phylogenetic analysis of full length S-segment open reading frame nucleotide sequences of hantaviruses in Paraguay in the context of the Americas. Abbreviations: Akodon montensis is Akmo; Oligoryzomys fornesi is Olfo.; Clades are grouped A, B, C; Viral strains abbreviations include Puumula (PUU), Prospect Hill (PH), Tula (TUL), Oro (ORO), Black Creek Canal (BCC), Muleshoe (MUL), Bayou (BAY), Catacamas (CAT), Limestone Canyon (LSC), El Moro Canyon (ELMC), Rio Segunda (RIOS), MGLV, New York (NY), Sin number (SN), Jabora virus (JAB), Ape aime (AAI), Bermejo (BML), (ORN), Andes (AND), Pergamino (PERG), Itapua (IP), Araraquara (ARA), Juquitiba (JUQ), Caño Delgadito (CAD), Choclo, Laguna Negra (LAN), Rio Mamoré (RIOM), Maporal.

Figure 2

Bayesian phylogenetic analysis of full length M-segment open reading frame nucleotide sequences of hantaviruses in Paraguay in the context of the Americas. Abbreviations: Akodon montensisis Akmo; Oligoryzomys fornesiis Olfo.; Clades are grouped A, B, C; Viral strains abbreviations include Puumula (PUU), Prospect Hill (PH), Tula (TUL), Oro (ORO), Black Creek Canal (BCC), Muleshoe (MUL), Bayou (BAY), Catacamas (CAT), Limestone Canyon (LSC), El Moro Canyon (ELMC), Rio Segunda (RIOS), MGLV, New York (NY), Sin number (SN), Jabora virus (JAB), Ape aime (AAI), Bermejo (BML), (ORN), Andes (AND), Pergamino (PERG), Itapua (IP), Araraquara (ARA), Juquitiba (JUQ), Caño Delgadito (CAD), Choclo, Laguna Negra (LAN), Rio Mamoré (RIOM), Maporal.