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. 2011;12(7):4522-35.
doi: 10.3390/ijms12074522. Epub 2011 Jul 13.

Antiviral activity and mechanism of action of novel thiourea containing chiral phosphonate on tobacco mosaic virus

Huitao Fan  1 , Baoan Song, Pinaki S Bhadury, Linhong Jin, Deyu Hu, Song Yang
Affiliations

Antiviral activity and mechanism of action of novel thiourea containing chiral phosphonate on tobacco mosaic virus

Huitao Fan et al. Int J Mol Sci. 2011.

Abstract

Using half-leaf method O,O'-diisopropyl (3-(L-1-(benzylamino)-1-oxo-3- phenylpropan-2-yl)thioureido)(phenyl)methyl phosphonate (2009104) was studied for its activity on tobacco mosaic virus (TMV). It showed good curative activity in vivo and the curative activity at 500 μg/mL was found to be 53.3%. In vivo treatment with the control agent Ningnanmycin at 500 μg/mL resulted in 51.2% inhibition and curative inhibition rates respectively. Dot-ELISA test was employed to verify the efficacy of activity of compound 200910 for anti-TMV activity. The mechanism of action of compound 2009104 to resist TMV was also studied. The results showed that the resistance enzymes PAL, POD, SOD activity and chlorophyll content after TMV inoculation K(326) (Nicotiana tabacum K(326)) of tobacco plants followed by treatment with compound 2009104 were significantly enhanced. The study of the effect of compound 2009104 on TMV capsid protein (CP) showed that it inhibited the polymerization process of TMV-CP in vitro.

Keywords: antiviral activity; chiral thiourea compounds; mechanism of action; tobacco mosaic virus.

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Figures

Figure 1
Figure 1
The molecular structure of 2009104.
Figure 2
Figure 2
The inhibitory effect of compound 2009104 on TMV in tobacco assayed by Dot-ELISA test.
Figure 3
Figure 3
PAL activity in tobacco leaves in differential treatment groups T1: CK; T2: TMV; T3: TMV+ Ningnanmycin; T4: TMV + 2009104.
Figure 4
Figure 4
POD activity in tobacco leaves in differential treatment groups T1: CK; T2: TMV; T3: TMV+ Ningnanmycin; T4: TMV+2009104.
Figure 5
Figure 5
SOD activity in tobacco leaves in differential treatment groups T1: CK; T2: TMV; T3: TMV+ Ningnanmycin; T4: TMV+2009104.
Figure 6
Figure 6
Changes of chlorophyll content in leaves treated with compound 2009104 T1: TMV; T2: CK; T3: TMV+Ningnanmycin; T4: TMV+2009104.
Figure 7
Figure 7
SDS-PAGE of TMV-CP Lane 1: Protein molecular weight marker; Lane 2: TMV-CP; Lane 3: TMV-CP.
Figure 8
Figure 8
UV (320 nm) absorption of TMV coat-protein polymerization in vitvo treated with compound 2009104 at different time intervals T1: TMV-CP; T2: 2009104 +TMV-CP; T3: Ribavirin +TMV-CP.
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