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. 2011 Sep;31(9):1957-62.
doi: 10.1161/ATVBAHA.110.220574.

Development of apolipoprotein E-deficient mice

Nobuyo Maeda  1
Affiliations

Development of apolipoprotein E-deficient mice

Nobuyo Maeda. Arterioscler Thromb Vasc Biol. 2011 Sep.
No abstract available

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Figures

Figure 1
Figure 1
Atherosclerotic plaques in one of our first apoE deficient mice sectioned by Bob Reddick. Panel A is a frozen section at the aortic root of a 9 month old male mouse stained with Sudan IVB and counterstained with hematoxylin showing plaques adherent to the valve attachment sites and vessel wall. Some areas of the plaque contain cholesterol clefts and fibrous caps while other areas are largely acellular. Panel B is a 1-μm thick section of a carotid artery from the same animal embedded in plastic and stained with Toluidine Blue, showing a plaque composed of foamy macrophages, cholesterol clefts, and fibrous protein deposits. A disruption of internal elastic lamina and the presence of smooth muscle cells with lipid droplets in both the plaque and media indicate smooth muscle cell proliferation, migration and lipid uptake. The mouse was on regular mouse chow.
See this image and copyright information in PMC

References

    1. Maeda N, Smithies O. The evolution of multigene families: human haptoglobin genes. Annu Rev Genet. 1986;20:81–108. - PubMed
    1. Smithies O, Gregg RG, Boggs SS, Koralewski MA, Kucherlapati RS. Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination. Nature. 1985;317(6034):230–234. - PubMed
    1. Thomas KR, Folger KR, Capecchi MR. High frequency targeting of genes to specific sites in the mammalian genome. Cell. 1986;44(3):419–428. - PubMed
    1. Gordon JW, Ruddle FH. Integration and stable germ line transmission of genes injected into mouse pronuclei. Science. 1981;214(4526):1244–1246. - PubMed
    1. Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature. 1981;292(5819):154–156. - PubMed

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